Ensuring the quality and safety of stem cells used in treatment involves a comprehensive approach encompassing stringent protocols and regulatory standards. Reputable clinics adhere to Good Manufacturing Practice (GMP) and Good Laboratory Practice (GLP) guidelines to maintain high-quality standards throughout the production process. This includes sourcing cells from certified, healthy donors, processing them in sterile environments, and conducting thorough testing for microbial contamination, endotoxins, and mycoplasma. Additionally, stem cells undergo cryopreservation to maintain their viability during storage and transport, with protocols in place to prevent damage during freezing and thawing . Before administration, cell viability and potency are assessed using validated methods like flow cytometry and phenotypic assays to ensure the cells’ functionality and therapeutic potential . Furthermore, adherence to Good Clinical Practice (GCP) ensures that clinical trials are conducted ethically and with patient safety as a priority . Clinics should also be registered with relevant regulatory bodies, such as the National Apex Committee for Stem Cell Research and Therapy (NAC-SCRT) in India, to ensure compliance with national standards . By inquiring about these measures, patients can assess the clinic’s commitment to providing safe and effective stem cell therapies.